Dark/Light Box

LSID: http://syli.cz/urn:lsid:public.sylics.com:conventionaltest:8A5D-2GA7-GE96

Materials and Methods
Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).

Associated Mammalian Phenotype Ontologies (MGI)
- MP:0001362 abnormal anxiety-related response
- MP:0001392 abnormal locomotor behaviour

Dark-light box
Mice were introduced into the dark compartment (<10lux, length x width x height: 25 x 25 x 30 cm) of a dark light box. 60 s later the motorized door opened providing access to an identical sized compartment which was brightly lit (625 lux) and left open for 10 min. Visits to, and time spent in the light compartment were counted when the body reference point of a mouse protruded at least 2 cm into the light compartment away from the door.

Parameter information
Parameter nameUnits
Distance moved in light compartmentcm
Average velocity in light compartmentcm/s
*Time spent in light compartmentSeconds
Activity in light compartment%
*Visits to light compartmentNumber
*Latency to visit the light compartmentSeconds
Distance moved in light compartment per minutecm
Time spent in light compartment per minuteSeconds
Visits in light compartment per minuteNumber
Distance moved in light compartment first and second five minutescm
Time spent in light compartment first and second five minutesSeconds
Visits in light compartment first and second five minutesNumber