LSID: http://syli.cz/urn:lsid:public.sylics.com:conventionaltest:796A-GD33-B8D4

Materials and Methods
Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).

Associated Mammalian Phenotype Ontologies (MGI)
- MP:0001447 abnormal nest building behavior

Nesting behavior
To assess nest building behavior, additional nesting material (Nestlet of 3 g compressed cotton; Ancare, Bellmore, NY) was introduced into each animal's home-cage approximately 3 hours before the start of the dark phase. The next morning, nest building behavior was scored according to a previously described rating scale of 1-5 (Weyer et al., 2011): 1 = Nestlet > 90% intact, 2 = Nestlet 50-90% intact, 3 = Nestlet mostly shredded but no identifiable nest site, 4 = identifiable but flat nest, 5 = crater-shaped nest.

Parameter information
Parameter nameUnits
*Nesting scoremanual
Nesting weightgram