Experiment name: Enhanced alcohol self-administration and reinstatement in a highly impulsive, inattentive recombinant inbred mouse strain. (Loos 2013)
LSID: http://syli.cz/urn:lsid:public.sylics.com:experiment:5B6A-9DB7-9724

TreatmentAmountAdministration routeAdministration time

Treatment info:
Order of behavioural testing
PhenoTyper Spontaneous Behaviour
PhenoTyper Avoidance Learning

Published in Frontiers in Behavioral Neuroscience 2013: http://journal.frontiersin.org/Journal/10.3389/fnbeh.2013.00151/full

Enhanced alcohol self-administration and reinstatement in a highly impulsive, inattentive recombinant inbred mouse strain.
Maarten Loos (1), Jorn Staal (1), August B. Smit (1), Taco J. De Vries (1,2) and Sabine Spijker (1)
1) Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University, Amsterdam, Netherlands, 2) Department of Anatomy and Neurosciences, Neuroscience Campus Amsterdam, VU University Medical Center, Amsterdam, Netherlands

Deficits in executive control have frequently been associated with alcohol use disorder. Here we investigated to what extent pre-existing genetically encoded levels of impulsive/inattentive behavior associate with motivation to take alcohol and vulnerability to cue-induced reinstatement of alcohol seeking in an operant self-administration paradigm. We took advantage of BXD16, a recombinant inbred strain previously shown to have enhanced impulsivity and poor attentional control. We compared BXD16 with C57BL/6J mice in a simple choice reaction time task (SCRTT) and confirmed its impulsive/inattentive phenotype. BXD16 mice were less active in a novel open field (OF), and were equally active in an automated home cage environment, showing that increased impulsive responding of BXD16 mice could not be explained by enhanced general activity compared to C57BL/6J mice. After training in a sucrose/alcohol fading self-administration procedure, BXD16 showed increased motivation to earn 10% alcohol solution, both under fixed ratio (FR1) and progressive ratio (PR2) schedules of reinforcement. Responding on the active lever readily decreased during extinction training with no apparent differences between strains. However, upon re-exposure to alcohol-associated cues, alcohol seeking was reinstated to a larger extent in BXD16 than in C57BL/6J mice. Although further studies are needed to determine whether impulsivity/inattention and alcohol seeking depend on common or separate genetic loci, these data show that in mice enhanced impulsivity coincides with increased motivation to take alcohol, as well as relapse vulnerability.

In this manuscript: Home-cage activity data of BXD16 mice
During the 3rd day in the automated home cage environment, i.e., after the effect of novelty on activity levels during the 1st days had largely faded (De Visser et al., 2006), activity of BXD16 was not different from C57BL/6J in terms of total distance moved during the dark phase (Figure 2A; ) or during the light phase.

Mouse info:
Mouse ID Strain Coat color Genotype Ear tag Internal ID Sex Date of Birth Sub experiment 1 Sub experiment 2 Sub experiment 3
PH00246 BXD16 unknown male 00-00-0000
PH00247 BXD16 unknown male 00-00-0000
PH00328 BXD16 unknown male 00-00-0000
PH00329 BXD16 unknown male 00-00-0000
PH00330 BXD16 unknown male 00-00-0000
PH00331 BXD16 unknown male 00-00-0000
PH01028 BXD16 unknown male 00-00-0000
PH01029 BXD16 unknown male 00-00-0000
PH01030 BXD16 unknown male 00-00-0000