Dark/Light Box
LSID: http://syli.cz/urn:lsid:public.sylics.com:conventionaltest:8A5D-2GA7-GE96Materials and Methods
Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).
Associated Mammalian Phenotype Ontologies (MGI)
- MP:0001362 abnormal anxiety-related response
- MP:0001392 abnormal locomotor behaviour
Dark-light box
Mice were introduced into the dark compartment (<10lux, length x width x height: 25 x 25 x 30 cm) of a dark light box. 60 s later the motorized door opened providing access to an identical sized compartment which was brightly lit (625 lux) and left open for 10 min. Visits to, and time spent in the light compartment were counted when the body reference point of a mouse protruded at least 2 cm into the light compartment away from the door.
Parameter information
| Parameter name | Units |
| Distance moved in light compartment | cm |
| Average velocity in light compartment | cm/s |
| *Time spent in light compartment | Seconds |
| Activity in light compartment | % |
| *Visits to light compartment | Number |
| *Latency to visit the light compartment | Seconds |
| Distance moved in light compartment per minute | cm |
| Time spent in light compartment per minute | Seconds |
| Visits in light compartment per minute | Number |
| Distance moved in light compartment first and second five minutes | cm |
| Time spent in light compartment first and second five minutes | Seconds |
| Visits in light compartment first and second five minutes | Number |