Novel Object Recognition
LSID: http://syli.cz/urn:lsid:public.sylics.com:conventionaltest:53AD-8B3E-B94EMaterials and Methods
Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).
Associated Mammalian Phenotype Ontologies (MGI)
- MP:0003461 abnormal response to novel object
- MP:0001362 abnormal anxiety-related response
- MP:0001392 abnormal locomotor behaviour
Novel object and place object task
The test was conducted in open field boxes, the day after the open field test. The testing day was divided into three phases: familiarization (F), novel place (NP) recognition and novel object (NO) recognition. During the initial familiarization stage, two identical objects were placed in the North and South corner of the arena equidistant (10 cm) from both walls. Each mouse was introduced in the West corner of the arena for 10 min to explore the objects and returned to the home cage afterwards. For NP recognition testing 2 hours later, mice were introduced for 5 min in the same arena with identical objects, of which one was placed in the East instead of the South corner. For NO recognition testing 2 hours after the NP testing, mice were introduced for 5 min in the same arena with a familiar object in the North corner and a novel object in the East corner. Object exploration was scored when the head of the animal was detected in a square zone (7.5 x 7.5 cm) containing the object, as detected by video tracking software (Viewer 2, BIOBSERVE GmbH, Bonn, Germany). The objects for a mouse to discriminate consisted of plastic Lego stacks of identical color but different shape (cube or pyramid). From mouse to mouse the color of the objects (red, yellow or blue) as well as the role of the pyramid shape (familiar or novel) were randomized and counterbalanced across genotypes. The arena and objects were cleaned with water and 70% ethanol after each trial. The basic measure was the time (in s) taken by the mice to explore the objects in the NP and NO trial. The performance was evaluated by calculating a discrimination index (N-F/N+F), where N = time spent exploring the object in the new location (NP) or the novel object (NO), F= time spent exploring the object in familiar location (NP) or familiar object (NO). The discrimination index was calculated for the first 2 minutes and the subsequent 2 minutes (i.e. min 3 and 4) after introduction into the arena.
Parameter information
| Parameter name | Units |
| Sample: Distance moved | cm |
| Sample: Velocity | cm/sec |
| Sample: Latency to first exploration of object | Seconds |
| Sample: Number of visits to object | Number |
| Sample: Distance moved per minute | cm |
| Sample: Visits to object 1 per minute | Number |
| Sample: Visits to object 2 per minute | Number |
| Sample: Visits preference index (min 1-2 vs 3-4) | Preference index |
| Sample: Duration to object 1 per minute | Seconds |
| Sample: Duration to object 2 per minute | Seconds |
| Sample: Duration preference index (min 1-2 vs 3-4) | Preference index |
| Place: Distance moved | cm |
| Place: Velocity | cm/sec |
| Place: Latency to first exploration of object | Seconds |
| Place: Number of visits to object | Number |
| Place: Distance moved per minute | cm |
| Place: Visits to object 1 per minute | Number |
| Place: Visits to object 2 per minute | Number |
| Place: Visits preference index (min 1-2 vs 3-4) | Preference index |
| Place: Duration to object 1 per minute | Seconds |
| Place: Duration to object 2 per minute | Seconds |
| Place: Duration preference index (min 1-2 vs 3-4) | Preference index |
| Object: Distance moved | cm |
| Object: Velocity | cm/sec |
| Object: Latency to first exploration of object | Seconds |
| Object: Number of visits to object | Number |
| Object: Distance moved per minute | cm |
| Object: Visits to object 1 per minute | Number |
| Object: Visits to object 2 per minute | Number |
| Object: Visits preference index (min 1-2 vs 3-4) | Preference index |
| Object: Duration to object 1 per minute | Seconds |
| Object: Duration to object 2 per minute | Seconds |
| Object: Duration preference index (min 1-2 vs 3-4) | Preference index |
| Object: Visits preference index (min 1-2) | Preference index |
| Object: Visits preference index (min 3-4) | Preference index |
| *Object: Duration preference index (min 1-2) | Preference index |
| Object: Duration preference index (min 3-4) | Preference index |
| Place: Visits preference index (min 1-2) | Preference index |
| Place: Visits preference index (min 3-4) | Preference index |
| *Place: Duration preference index (min 1-2) | Preference index |
| Place: Duration preference index (min 3-4) | Preference index |