Experiment name: MroH1 (Unpublished)
LSID: http://syli.cz/urn:lsid:public.sylics.com:experiment:5EA7-GBBE-GD83

Treatment	Amount	Administration route	Administration time

Treatment info:
Cohort(s)
Cohort 1, designated Batch 1 on the website:
Born between 14-nov-2013 and 16-nov-2013, from HZ x HZ breedings.
In total 18 WT and 18 HOM mice were selected.

Spontaneous behavior in the PhenoTyper
1 WT and 1 HOM mouse were marked as outside sleeper by the analysis. Data files of 16 WT and 15 HOM data files passed all Quality Control criteria. Video tracking was sub optimal (i.e. more than 5% of data missing) for 2 HOM mice.

There were no differences in any of the 20 key spontaneous behavior parameters. There were 2 marginally significant differences in the remaining 95 parameters.


Cognitive tests in the PhenoTyper

Avoidance learning (shelter task)
The PhenoTyper is equipped with a shelter compartment, with two entrances, in which mice typically spend 80% of their time (resting/sleeping). During the first 4 days, mice develop a preference to enter the shelter through one of the two entrances. The preference index is calculated by : [(number of entries through the preferred entrance) - (number of entries through
non-preferred entrance)]/(total number of entries).
Avoidance learning is studied by automatically applying a mild aversive stimulus (shelter illumination with bright light) during days 5 and 6 each time mice entered the shelter using their preferred entrance, but not when using the other entrance. A reduction in the preference index indicates that a mouse is establishing a specific association between its preferred entrance and the aversive stimulus. During day 7 sanctioning (i.e. shelter illumination) is discontinued, and the stability of the change in preference can be determined.
Avoidance learning is best studied during the Dark phase when shelter illumination is a stronger stimulus than during the light phase. For a detailed explanation see Maroteaux et al. (http://www.ncbi.nlm.nih.gov/pubmed/22846151)

Clear learning in both genotypes, no difference between genotypes.


Appetitive conditioning (pellet task)
Not performed

Anxiety test (Lightspot avoidance test)
Clear avoidance in both genotypes, no difference between genotypes.


Conventional tests

Not performed


Conclusions

A relatively large group of mice was tested, no behavioral differences were observed in the PhenoTyper. No conventional behavioral tests were scheduled.



Mouse info:

Mouse ID	Strain	Coat color	Genotype	Ear tag	Internal ID	Sex	Date of Birth	Sub experiment 1	Sub experiment 2	Sub experiment 3
PH08587	MroH1	black	hom	R	5887	male	16-11-2013
PH08588	MroH1	black	WT	L	5888	male	16-11-2013
PH08589	MroH1	black	WT	RL	5889	male	16-11-2013
PH08590	MroH1	black	hom	R	5896	male	14-11-2013
PH08591	MroH1	black	hom	L	5902	male	14-11-2013
PH08592	MroH1	black	hom	RR	5904	male	14-11-2013
PH08593	MroH1	black	WT	R	5912	male	14-11-2013
PH08594	MroH1	black	WT	R	5921	male	14-11-2013
PH08595	MroH1	black	hom	R	5928	male	14-11-2013
PH08596	MroH1	black	WT	L	5929	male	14-11-2013
PH08597	MroH1	black	hom	RR	5931	male	14-11-2013
PH08598	MroH1	black	hom	R	5941	male	14-11-2013
PH08599	MroH1	black	hom	RL	5943	male	14-11-2013
PH08600	MroH1	black	WT	LL	5945	male	14-11-2013
PH08601	MroH1	black	hom	R	5946	male	14-11-2013
PH08602	MroH1	black	hom	L	5947	male	14-11-2013
PH08603	MroH1	black	WT	RL	5948	male	14-11-2013
PH08604	MroH1	black	hom	RR	5949	male	14-11-2013
PH08605	MroH1	black	hom	RR	5963	male	14-11-2013
PH08606	MroH1	black	WT	RRL	5965	male	14-11-2013
PH08607	MroH1	black	WT	L	5967	male	14-11-2013
PH08608	MroH1	black	hom	RL	5973	male	14-11-2013
PH08609	MroH1	black	WT	RR	5974	male	14-11-2013
PH08610	MroH1	black	WT	R	5982	male	15-11-2013
PH08611	MroH1	black	WT	L	5983	male	15-11-2013
PH08612	MroH1	black	hom	RR	5985	male	15-11-2013
PH08613	MroH1	black	WT	R	5991	male	14-11-2013
PH08614	MroH1	black	hom	RL	5993	male	14-11-2013
PH08615	MroH1	black	WT	RR	5994	male	14-11-2013
PH08616	MroH1	black	WT	R	5999	male	14-11-2013
PH08617	MroH1	black	hom	L	6000	male	14-11-2013
PH08618	MroH1	black	WT	RL	6001	male	14-11-2013
PH08619	MroH1	black	WT	L	6024	male	14-11-2013
PH08620	MroH1	black	WT	RL	6025	male	14-11-2013
PH08621	MroH1	black	hom	RR	6026	male	14-11-2013
PH08622	MroH1	black	hom	RL	6032	male	14-11-2013