LSID: http://syli.cz/urn:lsid:public.sylics.com:experiment:24AD-G26D-G6BE
| Treatment | Amount | Administration route | Administration time |
Treatment info:
Order of behavioural testing
PhenoTyper Spontaneous Behaviour
PhenoTyper Appetitive Conditioning
PhenoTyper Avoidance Learning
Body Weight
Grip Strength
Vision Test
Nesting
Novel Home Cage Induced Hypophagia
Elevated Plus Maze
Open Field
Novel Object Recognition
T-Maze
Dark/Light Box
Rotorod
Barnes Maze
Fear Conditioning
Acoustic Startle Response and Prepulse Inhibition
Published in Brain Structure and Function 2014:
http://link.springer.com/article/10.1007%2Fs00429-014-0766-0
Tomosyn-2 is required for normal motor performance in mice and sustains neurotransmission at motor endplates
Cornelia J. Geerts1†, Jaap J. Plomp2†, Bastijn Koopmans3, Maarten Loos3, Lizette van der Pijl2, Martin A. van der Valk4, Matthijs Verhage1,5 and Alexander J.A. Groffen1,5
1 Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University, The Netherlands
2 Department of Molecular Cell Biology, Group Neurophysiology, Research Building, Leiden University Medical Centre, Leiden, The Netherlands
3 Sylics (Synaptologics BV), Amsterdam, The Netherlands
4 Department Experimental Animal Pathology, Antoni van Leeuwenhoek / Netherlands Cancer Institute, Amsterdam, The Netherlands
5 Department of Clinical Genetics, VU Medical Center, Amsterdam, Netherlands
† These authors contributed equally to this work
Abstract
Tomosyn-1 (STXBP5) is a soluble NSF attachment protein receptor complex-binding protein that inhibits vesicle fusion, but the role of tomosyn-2 (STXBP5L) in the mammalian nervous system is still unclear. Here we generated tomosyn-2 null (Tom2KO/KO) mice, which showed impaired motor performance. This was accompanied by synaptic changes at the neuromuscular junction, including enhanced spontaneous acetylcholine release frequency and faster depression of muscle motor endplate potentials during repetitive stimulation. The postsynaptic geometric arrangement and function of acetylcholine receptors were normal. We conclude that tomosyn-2 supports motor performance by regulation of transmitter release willingness to sustain synaptic strength during high-frequency transmission, which makes this gene a candidate for involvement in neuromuscular disorders.
Mouse info:
| Mouse ID | Strain | Coat color | Genotype | Ear tag | Internal ID | Sex | Date of Birth | Sub experiment 1 | Sub experiment 2 | Sub experiment 3 |
| PH07118 | Tom2(null) | black | hom | - | 6398 | male | 20-03-2013 | |||
| PH07119 | Tom2(null) | black | hom | R | 6399 | male | 20-03-2013 | |||
| PH07120 | Tom2(null) | black | WT | L | 6400 | male | 20-03-2013 | |||
| PH07121 | Tom2(null) | black | hom | RL | 6401 | male | 20-03-2013 | |||
| PH07122 | Tom2(null) | black | WT | RR | 6402 | male | 20-03-2013 | |||
| PH07123 | Tom2(null) | black | WT | LL | 6403 | male | 20-03-2013 | |||
| PH07124 | Tom2(null) | black | hom | - | 7093 | male | 05-04-2013 | |||
| PH07125 | Tom2(null) | black | hom | R | 7094 | male | 05-04-2013 | |||
| PH07126 | Tom2(null) | black | hom | L | 7095 | male | 05-04-2013 | |||
| PH07127 | Tom2(null) | black | WT | RL | 7096 | male | 05-04-2013 | |||
| PH07128 | Tom2(null) | black | WT | RR | 7097 | male | 05-04-2013 | |||
| PH07129 | Tom2(null) | black | WT | LL | 7098 | male | 05-04-2013 | |||
| PH07130 | Tom2(null) | black | hom | - | 7494 | male | 11-04-2013 | |||
| PH07131 | Tom2(null) | black | WT | R | 7495 | male | 11-04-2013 | |||
| PH07132 | Tom2(null) | black | WT | L | 7496 | male | 11-04-2013 | |||
| PH07133 | Tom2(null) | black | WT | RL | 7497 | male | 11-04-2013 | |||
| PH07134 | Tom2(null) | black | hom | RR | 7498 | male | 11-04-2013 | |||
| PH07135 | Tom2(null) | black | hom | LL | 7499 | male | 11-04-2013 |