LSID: http://syli.cz/urn:lsid:public.sylics.com:experiment:24AD-9246-E96B
| Treatment | Amount | Administration route | Administration time |
| BM reversal | |||
| Only LTP in paper |
Treatment info:
https://academic.oup.com/brain/article-lookup/doi/10.1093/brain/awy046
Protein instability, haploinsufficiency, and cortical hyperexcitability underlie STXBP1-encephalopathy seizures and cognitive deficits
Jovana Kovacevic1,3†, Gregoire Maroteaux1†, Desiree Schut2†, Maarten Loos3, Mohit Dubey4,
Julika Pitsch5, Esther Remmelink3, Bastijn Koopmans3, James Crowley6, L. Niels Cornelisse2,
Patrick F. Sullivan6,7, Susanne Schoch5, Ruud F. Toonen1, Oliver Stiedl1 and Matthijs Verhage1,2*
1 Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), VU University Amsterdam and VU Medical Center, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
2 Department of Clinical Genetics, Center for Neurogenomics and Cognitive Research (CNCR), VU University Amsterdam and VU Medical Center, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
3 Sylics (Synaptologics BV), Amsterdam, The Netherlands.
4 Department of Integrative Neurophysiology, Center for Neurogenomics and Cognitive Research (CNCR), VU University Amsterdam and VU Medical Center, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
5 Section for Translational Epilepsy Research, Department of Neuropathology, University of Bonn Medical Center, 53105 Bonn, Germany.
6 UNC Center for Psychiatric Genomics, University of North Carolina at Chapel Hill, 101 Manning Drive, Chapel Hill, NC 27599-7160, USA.
7 Karolinska Institutet, Department of Medical Epidemiology and Biostatistics 1 and Department of (Clinical) Genetics, Nobels väg 12A, 171 77 Stockholm, Sweden.
* corresponding author: Matthijs Verhage (email: matthijs@cncr.vu.nl)
Department of Functional Genomics, VU University Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
† shared first authorship, alphabetical order.
Abstract
De novo heterozygous mutations in STXBP1/Munc18-1 cause early infantile epileptic encephalopathies (EIEE4, OMIM #612164) characterized by infantile epilepsy, developmental delay, intellectual disability, and can include autistic features. We characterized the cellular deficits for an allelic series of seven STXBP1 mutations and developed four mouse models that recapitulate the abnormal EEG activity and cognitive aspects of human STXBP1-encephalopathy. Disease-causing STXBP1 variants supported synaptic transmission to a variable extent on a null background, but had no effect when overexpressed on a heterozygous background. All disease variants had severely decreased protein levels. Together, these cellular studies suggest that impaired protein stability and STXBP1 haploinsufficiency explain STXBP1-encephalopathy and that, therefore, Stxbp1+/- mice provide a valid mouse model. Simultaneous video- and EEG- recordings revealed that Stxbp1+/- mice with different genomic backgrounds recapitulate the seizure/spasm phenotype observed in humans, characterized by myoclonic jerks and spike-wave discharges that were suppressed by the antiepileptic drug levetiracetam. Mice heterozygous for Stxbp1 in GABA-ergic neurons only, showed impaired viability, 50% died within 2-3 weeks, and the rest showed stronger epileptic activity. c-Fos staining implicated neocortical areas, but not other brain regions, as the seizure foci. Stxbp1+/- mice showed impaired cognitive performance, hyperactivity and anxiety-like behavior, without altered social behavior. Taken together, these data demonstrate the construct, face and predictive validity of Stxbp1+/- mice and point to protein instability, haploinsufficiency and imbalanced excitation in neocortex, as the underlying mechanism of STXBP1-encephalopathy. The mouse models reported here are valid models for development of therapeutic interventions targeting STXBP1-encephalopathy.
Keywords: STXBP1-encephalopathy, haploinsufficiency, epilepsy, Video-EEG, behavioral inflexibility
Abbreviations: DIV = days in vitro; EPSC = evoked postsynaptic current; mCx = motor cortex; PFC = prefrontal cortex; RRP = readily releasable pool; ssCx = somatosensory cortex
DOI: 10.1093/brain/awy046.
Data not available in this repository:
Data acquired prior to 2011 is not available in this online repository. This data can be retreived from this public Google Drive folder:
http://syli.cz/kovacevicd
Mouse info:
| Mouse ID | Strain | Coat color | Genotype | Ear tag | Internal ID | Sex | Date of Birth | Sub experiment 1 | Sub experiment 2 | Sub experiment 3 |
| PH00343 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00344 | Munc18-1(BL6) | unknown | hetro | female | 00-00-0000 | |||||
| PH00345 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00346 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00347 | Munc18-1(BL6) | unknown | hetro | female | 00-00-0000 | |||||
| PH00348 | Munc18-1(BL6) | unknown | hetro | female | 00-00-0000 | |||||
| PH00349 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00350 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00351 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00353 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00354 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00355 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00356 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00357 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00358 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00359 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00875 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00876 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00877 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00878 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00879 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00880 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00881 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00882 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00883 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00884 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00885 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00886 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00888 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00889 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00890 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00891 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00892 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00893 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00894 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00895 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00896 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH00897 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH00898 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01135 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01136 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01137 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01138 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01141 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01142 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01143 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01144 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01145 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01147 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01148 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01149 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01150 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01151 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01152 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01154 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01155 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01156 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01157 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH01158 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH01159 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02253 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02254 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02255 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02256 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02257 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02259 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02260 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02261 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02262 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02263 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02264 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02265 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02266 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02267 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02268 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02269 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02270 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02271 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02272 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02274 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02275 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02276 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02277 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02305 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02306 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02307 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02308 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02309 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02310 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02311 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02312 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02313 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02314 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02315 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02317 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02318 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02319 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02320 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02321 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02322 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH02323 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH02324 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03598 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03599 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03600 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03601 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03602 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03603 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03604 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03605 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03606 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03607 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03608 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03609 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03610 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03611 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03612 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03613 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03614 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03615 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03616 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03617 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03618 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03619 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03620 | Munc18-1(BL6) | unknown | hetro | male | 00-00-0000 | |||||
| PH03621 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH03622 | Munc18-1(BL6) | unknown | WT | male | 00-00-0000 | |||||
| PH04314 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04315 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04316 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04317 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04318 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04319 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04320 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04321 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04322 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04323 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04324 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04325 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04326 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04327 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04328 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04329 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04330 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04331 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04332 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04333 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH04334 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04335 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04336 | Munc18-1(Floxed) | unknown | WT | male | 00-00-0000 | |||||
| PH04337 | Munc18-1(Floxed) | unknown | hetro | male | 00-00-0000 | |||||
| PH09078 | Munc18-1(129Sv) | black | hetro | L | 1439 | male | 09-01-2014 | |||
| PH09079 | Munc18-1(129Sv) | black | hetro | RL | 1460 | male | 09-01-2014 | |||
| PH09080 | Munc18-1(129Sv) | black | hetro | RR | 1461 | male | 09-01-2014 | |||
| PH09081 | Munc18-1(129Sv) | black | hetro | LL | 1462 | male | 09-01-2014 | |||
| PH09082 | Munc18-1(129Sv) | black | hetro | L | 1465 | male | 13-01-2014 | |||
| PH09083 | Munc18-1(129Sv) | black | hetro | RL | 1466 | male | 13-01-2014 | |||
| PH09084 | Munc18-1(129Sv) | black | WT | R | 1438 | male | 09-01-2014 | |||
| PH09085 | Munc18-1(129Sv) | black | WT | RL | 1440 | male | 09-01-2014 | |||
| PH09086 | Munc18-1(129Sv) | black | WT | R | 1445 | male | 09-01-2014 | |||
| PH09087 | Munc18-1(129Sv) | black | WT | L | 1446 | male | 09-01-2014 | |||
| PH09088 | Munc18-1(129Sv) | black | WT | RL | 1447 | male | 09-01-2014 | |||
| PH09089 | Munc18-1(129Sv) | black | WT | - | 1457 | male | 09-01-2014 | |||
| PH09090 | Munc18-1(129Sv) | black | WT | R | 1458 | male | 09-01-2014 | |||
| PH09091 | Munc18-1(129Sv) | black | WT | L | 1459 | male | 09-01-2014 | |||
| PH09092 | Munc18-1(129Sv) | black | WT | R | 1464 | male | 13-01-2014 | |||
| PH09093 | Munc18-1(129Sv) | black | hetro | R | 1471 | male | 15-01-2014 | |||
| PH09094 | Munc18-1(129Sv) | black | hetro | RL | 1473 | male | 15-01-2014 | |||
| PH09095 | Munc18-1(129Sv) | black | hetro | RR | 1474 | male | 15-01-2014 | |||
| PH09096 | Munc18-1(129Sv) | black | hetro | LL | 1475 | male | 15-01-2014 | |||
| PH09097 | Munc18-1(129Sv) | black | WT | R | 1479 | male | 16-01-2014 | |||
| PH09098 | Munc18-1(129Sv) | black | hetro | R | 1933 | male | 27-01-2014 | |||
| PH09099 | Munc18-1(129Sv) | black | hetro | L | 1934 | male | 27-01-2014 | |||
| PH09100 | Munc18-1(129Sv) | black | WT | RL | 1935 | male | 27-01-2014 | |||
| PH09101 | Munc18-1(129Sv) | black | WT | RR | 1936 | male | 27-01-2014 | |||
| PH13410 | Munc18-1(BL6) | black | hetro | 3068 | male | 05-02-2015 | Only LTP in paper | |||
| PH13411 | Munc18-1(BL6) | black | WT | 22644 | male | 05-02-2015 | Only LTP in paper | |||
| PH13412 | Munc18-1(BL6) | black | WT | 22645 | male | 05-02-2015 | Only LTP in paper | |||
| PH13413 | Munc18-1(BL6) | black | hetro | 22084 | male | 05-02-2015 | Only LTP in paper | |||
| PH13414 | Munc18-1(BL6) | black | WT | 22671 | male | 05-02-2015 | Only LTP in paper | |||
| PH13415 | Munc18-1(BL6) | black | WT | 22672 | male | 05-02-2015 | Only LTP in paper | |||
| PH13416 | Munc18-1(BL6) | black | hetro | 23526 | male | 05-02-2015 | Only LTP in paper | |||
| PH13417 | Munc18-1(BL6) | black | hetro | 23525 | male | 05-02-2015 | Only LTP in paper | |||
| PH13418 | Munc18-1(BL6) | black | WT | 22623 | male | 05-02-2015 | Only LTP in paper | |||
| PH13419 | Munc18-1(BL6) | black | hetro | 22629 | male | 05-02-2015 | Only LTP in paper | |||
| PH13420 | Munc18-1(BL6) | black | hetro | 22630 | male | 05-02-2015 | Only LTP in paper | |||
| PH13421 | Munc18-1(BL6) | black | WT | 22624 | male | 05-02-2015 | Only LTP in paper | |||
| PH13422 | Munc18-1(BL6) | black | hetro | 23163 | male | 05-02-2015 | Only LTP in paper | |||
| PH13423 | Munc18-1(BL6) | black | WT | 22584 | male | 05-02-2015 | Only LTP in paper | |||
| PH13424 | Munc18-1(BL6) | black | WT | 22585 | male | 05-02-2015 | Only LTP in paper | |||
| PH13425 | Munc18-1(BL6) | black | hetro | 22598 | male | 05-02-2015 | Only LTP in paper | |||
| PH13426 | Munc18-1(BL6) | black | WT | 22578 | male | 05-02-2015 | Only LTP in paper | |||
| PH13427 | Munc18-1(BL6) | black | WT | 22577 | male | 05-02-2015 | Only LTP in paper | |||
| PH13428 | Munc18-1(BL6) | black | hetro | 22600 | male | 05-02-2015 | Only LTP in paper | |||
| PH13429 | Munc18-1(BL6) | black | hetro | 22775 | male | 05-02-2015 | Only LTP in paper | |||
| PH13430 | Munc18-1(BL6) | black | WT | 22782 | male | 05-02-2015 | Only LTP in paper | |||
| PH13431 | Munc18-1(BL6) | black | hetro | 23162 | male | 05-02-2015 | Only LTP in paper | |||
| PH13432 | Munc18-1(BL6) | black | WT | 22783 | male | 05-02-2015 | Only LTP in paper | |||
| PH13433 | Munc18-1(BL6) | black | hetro | 23067 | male | 05-02-2015 | Only LTP in paper | |||
| PH13434 | Munc18-1(BL6) | black | hetro | 3068 | male | 05-02-2015 | ||||
| PH13435 | Munc18-1(BL6) | black | WT | 22644 | male | 05-02-2015 | ||||
| PH13436 | Munc18-1(BL6) | black | WT | 22645 | male | 05-02-2015 | ||||
| PH13437 | Munc18-1(BL6) | black | hetro | 22084 | male | 05-02-2015 | ||||
| PH13438 | Munc18-1(BL6) | black | WT | 22671 | male | 05-02-2015 | ||||
| PH13439 | Munc18-1(BL6) | black | WT | 22672 | male | 05-02-2015 | ||||
| PH13440 | Munc18-1(BL6) | black | hetro | 23526 | male | 05-02-2015 | ||||
| PH13441 | Munc18-1(BL6) | black | hetro | 23525 | male | 05-02-2015 | ||||
| PH13442 | Munc18-1(BL6) | black | WT | 22623 | male | 05-02-2015 | ||||
| PH13443 | Munc18-1(BL6) | black | hetro | 22629 | male | 05-02-2015 | ||||
| PH13444 | Munc18-1(BL6) | black | hetro | 22630 | male | 05-02-2015 | ||||
| PH13445 | Munc18-1(BL6) | black | WT | 22624 | male | 05-02-2015 | ||||
| PH13446 | Munc18-1(BL6) | black | hetro | 23163 | male | 05-02-2015 | ||||
| PH13447 | Munc18-1(BL6) | black | WT | 22584 | male | 05-02-2015 | ||||
| PH13448 | Munc18-1(BL6) | black | WT | 22585 | male | 05-02-2015 | ||||
| PH13449 | Munc18-1(BL6) | black | hetro | 22598 | male | 05-02-2015 | ||||
| PH13450 | Munc18-1(BL6) | black | WT | 22578 | male | 05-02-2015 | ||||
| PH13451 | Munc18-1(BL6) | black | WT | 22577 | male | 05-02-2015 | ||||
| PH13452 | Munc18-1(BL6) | black | hetro | 22600 | male | 05-02-2015 | ||||
| PH13453 | Munc18-1(BL6) | black | hetro | 22775 | male | 05-02-2015 | ||||
| PH13454 | Munc18-1(BL6) | black | WT | 22782 | male | 05-02-2015 | ||||
| PH13455 | Munc18-1(BL6) | black | hetro | 23162 | male | 05-02-2015 | ||||
| PH13456 | Munc18-1(BL6) | black | WT | 22783 | male | 05-02-2015 | ||||
| PH13457 | Munc18-1(BL6) | black | hetro | 23067 | male | 05-02-2015 | ||||
| PH13912 | Munc18-1(BL6) | black | hetro | 3068 | male | 05-02-2015 | BM reversal | |||
| PH13913 | Munc18-1(BL6) | black | WT | 22644 | male | 05-02-2015 | BM reversal | |||
| PH13914 | Munc18-1(BL6) | black | WT | 22645 | male | 05-02-2015 | BM reversal | |||
| PH13915 | Munc18-1(BL6) | black | hetro | 22084 | male | 05-02-2015 | BM reversal | |||
| PH13916 | Munc18-1(BL6) | black | WT | 22671 | male | 05-02-2015 | BM reversal | |||
| PH13917 | Munc18-1(BL6) | black | WT | 22672 | male | 05-02-2015 | BM reversal | |||
| PH13918 | Munc18-1(BL6) | black | hetro | 23526 | male | 05-02-2015 | BM reversal | |||
| PH13919 | Munc18-1(BL6) | black | hetro | 23525 | male | 05-02-2015 | BM reversal | |||
| PH13920 | Munc18-1(BL6) | black | WT | 22623 | male | 05-02-2015 | BM reversal | |||
| PH13921 | Munc18-1(BL6) | black | hetro | 22629 | male | 05-02-2015 | BM reversal | |||
| PH13922 | Munc18-1(BL6) | black | hetro | 22630 | male | 05-02-2015 | BM reversal | |||
| PH13923 | Munc18-1(BL6) | black | WT | 22624 | male | 05-02-2015 | BM reversal | |||
| PH13924 | Munc18-1(BL6) | black | hetro | 23163 | male | 05-02-2015 | BM reversal | |||
| PH13925 | Munc18-1(BL6) | black | WT | 22584 | male | 05-02-2015 | BM reversal | |||
| PH13926 | Munc18-1(BL6) | black | WT | 22585 | male | 05-02-2015 | BM reversal | |||
| PH13927 | Munc18-1(BL6) | black | hetro | 22598 | male | 05-02-2015 | BM reversal | |||
| PH13928 | Munc18-1(BL6) | black | WT | 22578 | male | 05-02-2015 | BM reversal | |||
| PH13929 | Munc18-1(BL6) | black | WT | 22577 | male | 05-02-2015 | BM reversal | |||
| PH13930 | Munc18-1(BL6) | black | hetro | 22600 | male | 05-02-2015 | BM reversal | |||
| PH13931 | Munc18-1(BL6) | black | hetro | 22775 | male | 05-02-2015 | BM reversal | |||
| PH13932 | Munc18-1(BL6) | black | WT | 22782 | male | 05-02-2015 | BM reversal | |||
| PH13933 | Munc18-1(BL6) | black | hetro | 23162 | male | 05-02-2015 | BM reversal | |||
| PH13934 | Munc18-1(BL6) | black | WT | 22783 | male | 05-02-2015 | BM reversal | |||
| PH13935 | Munc18-1(BL6) | black | hetro | 23067 | male | 05-02-2015 | BM reversal | |||
| PH15280 | Munc18-1(Floxed) | black | WT | 35980 | male | 25-07-2015 | ||||
| PH15281 | Munc18-1(Floxed) | black | WT | 36007 | male | 21-07-2015 | ||||
| PH15282 | Munc18-1(Floxed) | black | WT | 36022 | male | 25-07-2015 | ||||
| PH15283 | Munc18-1(Floxed) | black | WT | 36024 | male | 25-07-2015 | ||||
| PH15284 | Munc18-1(Floxed) | black | WT | 36025 | male | 25-07-2015 | ||||
| PH15285 | Munc18-1(Floxed) | black | WT | 36032 | male | 21-07-2015 | ||||
| PH15286 | Munc18-1(Floxed) | black | WT | 36033 | male | 21-07-2015 | ||||
| PH15287 | Munc18-1(Floxed) | black | WT | 36035 | male | 21-07-2015 | ||||
| PH15288 | Munc18-1(Floxed) | black | WT | 36048 | male | 22-07-2015 | ||||
| PH15289 | Munc18-1(Floxed) | black | WT | 36051 | male | 22-07-2015 | ||||
| PH15290 | Munc18-1(Floxed) | black | WT | 36053 | male | 22-07-2015 | ||||
| PH15291 | Munc18-1(Floxed) | black | WT | 36056 | male | 21-07-2015 | ||||
| PH15292 | Munc18-1(Floxed) | black | hetro | 35979 | male | 25-07-2015 | ||||
| PH15293 | Munc18-1(Floxed) | black | hetro | 36008 | male | 21-07-2015 | ||||
| PH15294 | Munc18-1(Floxed) | black | hetro | 36018 | male | 22-07-2015 | ||||
| PH15295 | Munc18-1(Floxed) | black | hetro | 36023 | male | 25-07-2015 | ||||
| PH15296 | Munc18-1(Floxed) | black | hetro | 36026 | male | 25-07-2015 | ||||
| PH15297 | Munc18-1(Floxed) | black | hetro | 36031 | male | 21-07-2015 | ||||
| PH15298 | Munc18-1(Floxed) | black | hetro | 36034 | male | 21-07-2015 | ||||
| PH15299 | Munc18-1(Floxed) | black | hetro | 36036 | male | 21-07-2015 | ||||
| PH15300 | Munc18-1(Floxed) | black | hetro | 36049 | male | 22-07-2015 | ||||
| PH15301 | Munc18-1(Floxed) | black | hetro | 36050 | male | 22-07-2015 | ||||
| PH15302 | Munc18-1(Floxed) | black | hetro | 36052 | male | 22-07-2015 | ||||
| PH15303 | Munc18-1(Floxed) | black | hetro | 36057 | male | 21-07-2015 | ||||
| PH15304 | Munc18-1(Floxed) | black | WT | 35980 | male | 25-07-2015 | ||||
| PH15305 | Munc18-1(Floxed) | black | WT | 36007 | male | 21-07-2015 | ||||
| PH15306 | Munc18-1(Floxed) | black | WT | 36022 | male | 25-07-2015 | ||||
| PH15307 | Munc18-1(Floxed) | black | WT | 36024 | male | 25-07-2015 | ||||
| PH15308 | Munc18-1(Floxed) | black | WT | 36025 | male | 25-07-2015 | ||||
| PH15309 | Munc18-1(Floxed) | black | WT | 36032 | male | 21-07-2015 | ||||
| PH15310 | Munc18-1(Floxed) | black | WT | 36033 | male | 21-07-2015 | ||||
| PH15311 | Munc18-1(Floxed) | black | WT | 36035 | male | 21-07-2015 | ||||
| PH15312 | Munc18-1(Floxed) | black | WT | 36048 | male | 22-07-2015 | ||||
| PH15313 | Munc18-1(Floxed) | black | WT | 36051 | male | 22-07-2015 | ||||
| PH15314 | Munc18-1(Floxed) | black | WT | 36053 | male | 22-07-2015 | ||||
| PH15315 | Munc18-1(Floxed) | black | WT | 36056 | male | 21-07-2015 | ||||
| PH15316 | Munc18-1(Floxed) | black | hetro | 35979 | male | 25-07-2015 | ||||
| PH15317 | Munc18-1(Floxed) | black | hetro | 36008 | male | 21-07-2015 | ||||
| PH15318 | Munc18-1(Floxed) | black | hetro | 36018 | male | 22-07-2015 | ||||
| PH15319 | Munc18-1(Floxed) | black | hetro | 36023 | male | 25-07-2015 | ||||
| PH15320 | Munc18-1(Floxed) | black | hetro | 36026 | male | 25-07-2015 | ||||
| PH15321 | Munc18-1(Floxed) | black | hetro | 36031 | male | 21-07-2015 | ||||
| PH15322 | Munc18-1(Floxed) | black | hetro | 36034 | male | 21-07-2015 | ||||
| PH15323 | Munc18-1(Floxed) | black | hetro | 36036 | male | 21-07-2015 | ||||
| PH15324 | Munc18-1(Floxed) | black | hetro | 36049 | male | 22-07-2015 | ||||
| PH15325 | Munc18-1(Floxed) | black | hetro | 36050 | male | 22-07-2015 | ||||
| PH15326 | Munc18-1(Floxed) | black | hetro | 36052 | male | 22-07-2015 | ||||
| PH15327 | Munc18-1(Floxed) | black | hetro | 36057 | male | 21-07-2015 |