CatWalk: Interlimb coordination

LSID: http://syli.cz/urn:lsid:public.sylics.com:automatedtest:BE47-2548-DG8A

Materials and Methods
Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).

Activity in the home cage was automatically recorded by video tracking in specially designed cages (PhenoTyper model 3000, Noldus Information Technology, www.noldus.com/phenotyper). Each cage contains a top unit with built-in hardware for video-tracking, that is, an infrared-sensitive video camera. The latter provide constant and even illumination of the cage. An infrared filter placed in front of the camera prevents interference with room illumination. This method allows continuous behavioural recordings in both dark and light periods. EthoVision was used as video tracking and trial control software. PhenoTyper cages were connected in a specially designed computer network. The cages (L =30 × W =30 × H =35 cm) were made of transparent Perspex walls with an opaque Perspex floor covered with bedding based on cellulose. A feeding station and a water bottle were attached on to two adjacent walls outside of the cage. A shelter (height: 10 cm, diameter: 9 cm; non-transparent material) was fixed in one of the corners. The X-Y coordinates of the centre of gravity of mice were acquired and smoothed using EthoVision software and processed to generate behavioural parameters using AHCODA analysis software (Synaptologics BV, Amsterdam, The Netherlands, http://www.sylics.com/bioinformatics/ahcodatm-data-analysis/).

Parameter information
Parameter nameUnitsExplanation
All - Step Sequence Alternate (AA)Percentage
All - Step Sequence Alternate (AB)Percentage
All - Step Sequence Cruciate (A)Percentage
All - Step Sequence Cruciate (B)Percentage
All - Step Sequence PatternsCount
All - Step Sequence Regularity IndexPercentage
All - Step Sequence Rotate (A)Percentage
All - Step Sequence Rotate (B)Percentage
All - Support diagonalPercentage
All - Support fourPercentage
All - Support girdlePercentage
All - Support lateralPercentage
All - Support singlePercentage
All - Support threePercentage
All - Support zeroPercentage
FP - Base of SupportDistance (cm)
FP - Stride lengthDistance (cm)
HP - Base of SupportDistance (cm)
HP - Stride lengthDistance (cm)
Left - Mean print positionsDistance (cm)
LF - Stride lengthDistance (cm)
LF-LH - CouplingsPercentage
LF-LH - Couplings CstatPercentage
LF-LH - Couplings Cstat-RPercentage
LF-LH - Phase DispersionPercentage
LF-LH - Phase Lag CstatPercentage
LF-LH - Phase Lag Cstat-RPercentage
LF-RF - CouplingsPercentage
LF-RF - Couplings CstatPercentage
LF-RF - Couplings Cstat-RPercentage
LF-RF - Phase DispersionPercentage
LF-RF - Phase Lag CstatPercentage
LF-RF - Phase Lag Cstat-RPercentage
LF-RH - CouplingsPercentage
LF-RH - Couplings CstatPercentage
LF-RH - Couplings Cstat-RPercentage
LF-RH - Phase DispersionPercentage
LF-RH - Phase Lag CstatPercentage
LF-RH - Phase Lag Cstat-RPercentage
LH - Stride lengthDistance (cm)
LH-LF - CouplingsPercentage
LH-LF - Couplings CstatPercentage
LH-LF - Couplings Cstat-RPercentage
LH-RF - CouplingsPercentage
LH-RF - Couplings CstatPercentage
LH-RF - Couplings Cstat-RPercentage
LH-RH - CouplingsPercentage
LH-RH - Couplings CstatPercentage
LH-RH - Couplings Cstat-RPercentage
LH-RH - Phase DispersionPercentage
LH-RH - Phase Lag CstatPercentage
LH-RH - Phase Lag Cstat-RPercentage
RF - Stride lengthDistance (cm)
RF-LF - CouplingsPercentage
RF-LF - Couplings CstatPercentage
RF-LF - Couplings Cstat-RPercentage
RF-LH - CouplingsPercentage
RF-LH - Couplings CstatPercentage
RF-LH - Couplings Cstat-RPercentage
RF-LH - Phase DispersionPercentage
RF-LH - Phase Lag CstatPercentage
RF-LH - Phase Lag Cstat-RPercentage
RF-RH - CouplingsPercentage
RF-RH - Couplings CstatPercentage
RF-RH - Couplings Cstat-RPercentage
RF-RH - Phase DispersionPercentage
RF-RH - Phase Lag CstatPercentage
RF-RH - Phase Lag Cstat-RPercentage
RH - Stride lengthDistance (cm)
RH-LF - CouplingsPercentage
RH-LF - Couplings CstatPercentage
RH-LF - Couplings Cstat-RPercentage
RH-LH - CouplingsPercentage
RH-LH - Couplings CstatPercentage
RH-LH - Couplings Cstat-RPercentage
RH-RF - CouplingsPercentage
RH-RF - Couplings CstatPercentage
RH-RF - Couplings Cstat-RPercentage
Right - Mean print positionsDistance (cm)