## CatWalk: Interlimb coordination

**LSID: http://syli.cz/urn:lsid:public.sylics.com:automatedtest:BE47-2548-DG8A**

**Materials and Methods**

Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).

Activity in the home cage was automatically recorded by video tracking in specially designed cages (PhenoTyper model 3000, Noldus Information Technology, www.noldus.com/phenotyper). Each cage contains a top unit with built-in hardware for video-tracking, that is, an infrared-sensitive video camera. The latter provide constant and even illumination of the cage. An infrared filter placed in front of the camera prevents interference with room illumination. This method allows continuous behavioural recordings in both dark and light periods. EthoVision was used as video tracking and trial control software. PhenoTyper cages were connected in a specially designed computer network. The cages (L =30 × W =30 × H =35 cm) were made of transparent Perspex walls with an opaque Perspex floor covered with bedding based on cellulose. A feeding station and a water bottle were attached on to two adjacent walls outside of the cage. A shelter (height: 10 cm, diameter: 9 cm; non-transparent material) was fixed in one of the corners. The X-Y coordinates of the centre of gravity of mice were acquired and smoothed using EthoVision software and processed to generate behavioural parameters using AHCODA analysis software (Synaptologics BV, Amsterdam, The Netherlands, http://www.sylics.com/bioinformatics/ahcodatm-data-analysis/).

**Parameter information**

Parameter name | Units | Explanation |

All - Step Sequence Alternate (AA) | Percentage | |

All - Step Sequence Alternate (AB) | Percentage | |

All - Step Sequence Cruciate (A) | Percentage | |

All - Step Sequence Cruciate (B) | Percentage | |

All - Step Sequence Patterns | Count | |

All - Step Sequence Regularity Index | Percentage | |

All - Step Sequence Rotate (A) | Percentage | |

All - Step Sequence Rotate (B) | Percentage | |

All - Support diagonal | Percentage | |

All - Support four | Percentage | |

All - Support girdle | Percentage | |

All - Support lateral | Percentage | |

All - Support single | Percentage | |

All - Support three | Percentage | |

All - Support zero | Percentage | |

FP - Base of Support | Distance (cm) | |

FP - Stride length | Distance (cm) | |

HP - Base of Support | Distance (cm) | |

HP - Stride length | Distance (cm) | |

Left - Mean print positions | Distance (cm) | |

LF - Stride length | Distance (cm) | |

LF-LH - Couplings | Percentage | |

LF-LH - Couplings Cstat | Percentage | |

LF-LH - Couplings Cstat-R | Percentage | |

LF-LH - Phase Dispersion | Percentage | |

LF-LH - Phase Lag Cstat | Percentage | |

LF-LH - Phase Lag Cstat-R | Percentage | |

LF-RF - Couplings | Percentage | |

LF-RF - Couplings Cstat | Percentage | |

LF-RF - Couplings Cstat-R | Percentage | |

LF-RF - Phase Dispersion | Percentage | |

LF-RF - Phase Lag Cstat | Percentage | |

LF-RF - Phase Lag Cstat-R | Percentage | |

LF-RH - Couplings | Percentage | |

LF-RH - Couplings Cstat | Percentage | |

LF-RH - Couplings Cstat-R | Percentage | |

LF-RH - Phase Dispersion | Percentage | |

LF-RH - Phase Lag Cstat | Percentage | |

LF-RH - Phase Lag Cstat-R | Percentage | |

LH - Stride length | Distance (cm) | |

LH-LF - Couplings | Percentage | |

LH-LF - Couplings Cstat | Percentage | |

LH-LF - Couplings Cstat-R | Percentage | |

LH-RF - Couplings | Percentage | |

LH-RF - Couplings Cstat | Percentage | |

LH-RF - Couplings Cstat-R | Percentage | |

LH-RH - Couplings | Percentage | |

LH-RH - Couplings Cstat | Percentage | |

LH-RH - Couplings Cstat-R | Percentage | |

LH-RH - Phase Dispersion | Percentage | |

LH-RH - Phase Lag Cstat | Percentage | |

LH-RH - Phase Lag Cstat-R | Percentage | |

RF - Stride length | Distance (cm) | |

RF-LF - Couplings | Percentage | |

RF-LF - Couplings Cstat | Percentage | |

RF-LF - Couplings Cstat-R | Percentage | |

RF-LH - Couplings | Percentage | |

RF-LH - Couplings Cstat | Percentage | |

RF-LH - Couplings Cstat-R | Percentage | |

RF-LH - Phase Dispersion | Percentage | |

RF-LH - Phase Lag Cstat | Percentage | |

RF-LH - Phase Lag Cstat-R | Percentage | |

RF-RH - Couplings | Percentage | |

RF-RH - Couplings Cstat | Percentage | |

RF-RH - Couplings Cstat-R | Percentage | |

RF-RH - Phase Dispersion | Percentage | |

RF-RH - Phase Lag Cstat | Percentage | |

RF-RH - Phase Lag Cstat-R | Percentage | |

RH - Stride length | Distance (cm) | |

RH-LF - Couplings | Percentage | |

RH-LF - Couplings Cstat | Percentage | |

RH-LF - Couplings Cstat-R | Percentage | |

RH-LH - Couplings | Percentage | |

RH-LH - Couplings Cstat | Percentage | |

RH-LH - Couplings Cstat-R | Percentage | |

RH-RF - Couplings | Percentage | |

RH-RF - Couplings Cstat | Percentage | |

RH-RF - Couplings Cstat-R | Percentage | |

Right - Mean print positions | Distance (cm) |