AHCODA-DB


Lightspot test (1 day)

LSID: http://syli.cz/urn:lsid:public.sylics.com:automatedtest:89GE-DEB7-2B67

Materials and Methods
Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).

Activity in the home cage was automatically recorded by video tracking in specially designed cages (PhenoTyper model 3000, Noldus Information Technology, www.noldus.com/phenotyper). Each cage contains a top unit with built-in hardware for video-tracking, that is, an infrared-sensitive video camera. The latter provide constant and even illumination of the cage. An infrared filter placed in front of the camera prevents interference with room illumination. This method allows continuous behavioural recordings in both dark and light periods. EthoVision was used as video tracking and trial control software. PhenoTyper cages were connected in a specially designed computer network. The cages (L =30 × W =30 × H =35 cm) were made of transparent Perspex walls with an opaque Perspex floor covered with bedding based on cellulose. A feeding station and a water bottle were attached on to two adjacent walls outside of the cage. A shelter (height: 10 cm, diameter: 9 cm; non-transparent material) was fixed in one of the corners. The X-Y coordinates of the centre of gravity of mice were acquired and smoothed using EthoVision software and processed to generate behavioural parameters using AHCODA analysis software (Synaptologics BV, Amsterdam, The Netherlands, http://www.sylics.com/bioinformatics/ahcodatm-data-analysis/).

Lightspot test

Parameter information
Parameter nameUnitsExplanation
Time Outside (Q1-Q6)sThe time the animal spent outside the shelter between Q1 (the quarter before the lightspot switched on) and Q6 (the quarter after the lightspot switched off)
Time in Feeder (Q1-Q6)sThe time the animal spent in the Feeder area between Q1 (the quarter before the lightspot switched on) and Q6 (the quarter after the lightspot switched off)
Time in Shelter (Q1-Q6)sThe time the animal spent in the Shelter between Q1 (the quarter before the lightspot switched on) and Q6 (the quarter after the lightspot switched off)
Time Outside (Q2-Q5)sThe time the animal spent outside the shelter between Q2 (the quarter the lightspot switched on) and Q5 (the last quarter the lightspot is on)
Time in Feeder (Q2-Q5)sThe time the animal spent in the Feeder area between Q2 (the quarter the lightspot switched on) and Q5 (the last quarter the lightspot is on)
Time in Shelter (Q2-Q5)sThe time the animal spent in the Shelter between Q2 (the quarter the lightspot switched on) and Q5 (the last quarter the lightspot is on)