Open Field (10 minutes)

LSID: http://syli.cz/urn:lsid:public.sylics.com:conventionaltest:4EA3-2B58-G659

Materials and Methods
Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).

Associated Mammalian Phenotype Ontologies (MGI)
- MP:0001362 abnormal anxiety-related response
- MP:0001392 abnormal locomotor behaviour
- MP:0003492 abnormal involuntary movement

Open field
Mice were introduced into a corner of a white square open field (50 x 50 cm, walls 35 cm high) illuminated with a single white fluorescent light bulb from above (200 lx) and exploration was tracked for 10 min (Viewer 2, BIOBSERVE GmbH, Bonn, Germany). The surface area was divided into nine equally sized squares, and the center square was used as center area. Zone visits to the center area were counted using the body reference point. To counteract the detection of distance moved due to jitter of body reference point produced by grainy video signal, track correction option was set to 1.

Parameter information
Parameter nameUnits
*Total distance movedcm
Average velocitycm/s
Distance moved in centercm
*Time spent in centerSeconds
Latency to enter the centerSeconds
Visits in centerNumber
Distance moved first and second five minutescm
Average velocity first and second five minutescm/sec
Distance moved in center first and second five minutescm
Time spent in center first and second five minutesSeconds
Visits in center first and second five minutesNumber