Elevated Plus Maze (5 min)

LSID: http://syli.cz/urn:lsid:public.sylics.com:conventionaltest:D4A7-EGED-B982

Materials and Methods
Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).

Associated Mammalian Phenotype Ontologies (MGI)
- MP:0001362 abnormal anxiety-related response
- MP:0001392 abnormal locomotor behaviour

Elevated plus maze
Mice were introduced onto the center of an EPM facing a closed arm (arms 30 x 6 cm, walls 35 cm high, elevated 50 cm above the ground). The EPM was illuminated with a single white fluorescent light bulb from above (open arms 70 lx, closed arm 30 lx) and exploratory behavior was video tracked for 5 min (Viewer 2, BIOBSERVE GmbH, Bonn, Germany). The border between center and arm entries was defined at 3 cm into each arm. Zone visits were analyzed using the elevated plus maze plugin of the tracking software, which was set to count zone visits if both the nose and body reference point had crossed the zone a zone border. Dependant measures were the number of open arm visits, time spent on the open arms and the first latency to explore an open arm. To counteract the detection of distance moved due to jitter of body reference point produced by grainy video signal, track correction option was set to 1.

Parameter information
Parameter nameUnits
Latency to enter the open armsSeconds
Visits in open armsNumber
*Visits to open arms (percentage)Percent
*Time spent in open arms (s)Seconds
Distance moved in open armscm
Distance moved in open arms (percentage)Percent
Visits in closed armsNumber
Visits in closed arms (percentage)Percent
Time spent in closed armsSeconds
Distance moved in closed armscm
Distance moved in closed arms (percentage)Percent
Time spent in centerSeconds
Distance moved in centercm
Distance moved in center (percentage)Percent
*Total distance movedcm