Neurological score (long-term)

LSID: http://syli.cz/urn:lsid:public.sylics.com:conventionaltest:3982-B68E-B7AD

Materials and Methods
Mice were singly housed on sawdust in standard Makrolon type II cages enriched with cardboard nesting material for at least one week prior to experiments, with water and food ad libitum (7:00/19:00 lights on/off; providing an abrupt phase transition).

Associated Mammalian Phenotype Ontologies (MGI)
- MP:0000745 tremors

Neurological score
General wellbeing of the mice was assessed by observation of a number of characteristics.

0) Full extension of hind legs away from lateral midline when mouse is suspended by its tail, and mouse can hold this for 2 seconds, suspended 23 times.
1) Collapse or partial collapse of leg extension towards lateral midline (weakness) or trembling of hind legs during tail suspension.
2) Toes curl under at least twice during walking of 12 inches, or any part of foot is dragging along cage bottom/table (food provided in cage floor).
3) Rigid paralysis or minimal joint movement, foot not being used for forward motion.
4) Mouse cannot right itself within 30 seconds from either side.

Parameter information
Parameter nameUnits
*Neurological scoreScore