AHCODA-DB

Experiment name: Kovacevic2017
LSID: http://syli.cz/urn:lsid:public.sylics.com:experiment:24AD-9246-E96B

TreatmentAmountAdministration routeAdministration time
BM reversal
Only LTP in paper


Treatment info:
https://academic.oup.com/brain/article-lookup/doi/10.1093/brain/awy046

Protein instability, haploinsufficiency, and cortical hyperexcitability underlie STXBP1-encephalopathy seizures and cognitive deficits

Jovana Kovacevic1,3, Gregoire Maroteaux1, Desiree Schut2, Maarten Loos3, Mohit Dubey4,
Julika Pitsch5, Esther Remmelink3, Bastijn Koopmans3, James Crowley6, L. Niels Cornelisse2,
Patrick F. Sullivan6,7, Susanne Schoch5, Ruud F. Toonen1, Oliver Stiedl1 and Matthijs Verhage1,2*

1 Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), VU University Amsterdam and VU Medical Center, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
2 Department of Clinical Genetics, Center for Neurogenomics and Cognitive Research (CNCR), VU University Amsterdam and VU Medical Center, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
3 Sylics (Synaptologics BV), Amsterdam, The Netherlands.
4 Department of Integrative Neurophysiology, Center for Neurogenomics and Cognitive Research (CNCR), VU University Amsterdam and VU Medical Center, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
5 Section for Translational Epilepsy Research, Department of Neuropathology, University of Bonn Medical Center, 53105 Bonn, Germany.
6 UNC Center for Psychiatric Genomics, University of North Carolina at Chapel Hill, 101 Manning Drive, Chapel Hill, NC 27599-7160, USA.
7 Karolinska Institutet, Department of Medical Epidemiology and Biostatistics 1 and Department of (Clinical) Genetics, Nobels väg 12A, 171 77 Stockholm, Sweden.

* corresponding author: Matthijs Verhage (email: matthijs@cncr.vu.nl)
Department of Functional Genomics, VU University Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
shared first authorship, alphabetical order.

Abstract
De novo heterozygous mutations in STXBP1/Munc18-1 cause early infantile epileptic encephalopathies (EIEE4, OMIM #612164) characterized by infantile epilepsy, developmental delay, intellectual disability, and can include autistic features. We characterized the cellular deficits for an allelic series of seven STXBP1 mutations and developed four mouse models that recapitulate the abnormal EEG activity and cognitive aspects of human STXBP1-encephalopathy. Disease-causing STXBP1 variants supported synaptic transmission to a variable extent on a null background, but had no effect when overexpressed on a heterozygous background. All disease variants had severely decreased protein levels. Together, these cellular studies suggest that impaired protein stability and STXBP1 haploinsufficiency explain STXBP1-encephalopathy and that, therefore, Stxbp1+/- mice provide a valid mouse model. Simultaneous video- and EEG- recordings revealed that Stxbp1+/- mice with different genomic backgrounds recapitulate the seizure/spasm phenotype observed in humans, characterized by myoclonic jerks and spike-wave discharges that were suppressed by the antiepileptic drug levetiracetam. Mice heterozygous for Stxbp1 in GABA-ergic neurons only, showed impaired viability, 50% died within 2-3 weeks, and the rest showed stronger epileptic activity. c-Fos staining implicated neocortical areas, but not other brain regions, as the seizure foci. Stxbp1+/- mice showed impaired cognitive performance, hyperactivity and anxiety-like behavior, without altered social behavior. Taken together, these data demonstrate the construct, face and predictive validity of Stxbp1+/- mice and point to protein instability, haploinsufficiency and imbalanced excitation in neocortex, as the underlying mechanism of STXBP1-encephalopathy. The mouse models reported here are valid models for development of therapeutic interventions targeting STXBP1-encephalopathy.

Keywords: STXBP1-encephalopathy, haploinsufficiency, epilepsy, Video-EEG, behavioral inflexibility
Abbreviations: DIV = days in vitro; EPSC = evoked postsynaptic current; mCx = motor cortex; PFC = prefrontal cortex; RRP = readily releasable pool; ssCx = somatosensory cortex

DOI: 10.1093/brain/awy046.

Data not available in this repository:
Data acquired prior to 2011 is not available in this online repository. This data can be retreived from this public Google Drive folder:
http://syli.cz/kovacevicd


Mouse info:
Mouse ID Strain Coat color Genotype Ear tag Internal ID Sex Date of Birth Sub experiment 1 Sub experiment 2 Sub experiment 3
PH00343 Munc18-1(BL6) unknown WT male 00-00-0000
PH00344 Munc18-1(BL6) unknown hetro female 00-00-0000
PH00345 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00346 Munc18-1(BL6) unknown WT male 00-00-0000
PH00347 Munc18-1(BL6) unknown hetro female 00-00-0000
PH00348 Munc18-1(BL6) unknown hetro female 00-00-0000
PH00349 Munc18-1(BL6) unknown WT male 00-00-0000
PH00350 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00351 Munc18-1(BL6) unknown WT male 00-00-0000
PH00353 Munc18-1(BL6) unknown WT male 00-00-0000
PH00354 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00355 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00356 Munc18-1(BL6) unknown WT male 00-00-0000
PH00357 Munc18-1(BL6) unknown WT male 00-00-0000
PH00358 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00359 Munc18-1(BL6) unknown WT male 00-00-0000
PH00875 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00876 Munc18-1(BL6) unknown WT male 00-00-0000
PH00877 Munc18-1(BL6) unknown WT male 00-00-0000
PH00878 Munc18-1(BL6) unknown WT male 00-00-0000
PH00879 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00880 Munc18-1(BL6) unknown WT male 00-00-0000
PH00881 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00882 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00883 Munc18-1(BL6) unknown WT male 00-00-0000
PH00884 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00885 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00886 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00888 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00889 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00890 Munc18-1(BL6) unknown WT male 00-00-0000
PH00891 Munc18-1(BL6) unknown WT male 00-00-0000
PH00892 Munc18-1(BL6) unknown WT male 00-00-0000
PH00893 Munc18-1(BL6) unknown WT male 00-00-0000
PH00894 Munc18-1(BL6) unknown WT male 00-00-0000
PH00895 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00896 Munc18-1(BL6) unknown hetro male 00-00-0000
PH00897 Munc18-1(BL6) unknown WT male 00-00-0000
PH00898 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01135 Munc18-1(BL6) unknown WT male 00-00-0000
PH01136 Munc18-1(BL6) unknown WT male 00-00-0000
PH01137 Munc18-1(BL6) unknown WT male 00-00-0000
PH01138 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01141 Munc18-1(BL6) unknown WT male 00-00-0000
PH01142 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01143 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01144 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01145 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01147 Munc18-1(BL6) unknown WT male 00-00-0000
PH01148 Munc18-1(BL6) unknown WT male 00-00-0000
PH01149 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01150 Munc18-1(BL6) unknown WT male 00-00-0000
PH01151 Munc18-1(BL6) unknown WT male 00-00-0000
PH01152 Munc18-1(BL6) unknown WT male 00-00-0000
PH01154 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01155 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01156 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01157 Munc18-1(BL6) unknown WT male 00-00-0000
PH01158 Munc18-1(BL6) unknown hetro male 00-00-0000
PH01159 Munc18-1(BL6) unknown WT male 00-00-0000
PH02253 Munc18-1(BL6) unknown WT male 00-00-0000
PH02254 Munc18-1(BL6) unknown WT male 00-00-0000
PH02255 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02256 Munc18-1(BL6) unknown WT male 00-00-0000
PH02257 Munc18-1(BL6) unknown WT male 00-00-0000
PH02259 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02260 Munc18-1(BL6) unknown WT male 00-00-0000
PH02261 Munc18-1(BL6) unknown WT male 00-00-0000
PH02262 Munc18-1(BL6) unknown WT male 00-00-0000
PH02263 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02264 Munc18-1(BL6) unknown WT male 00-00-0000
PH02265 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02266 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02267 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02268 Munc18-1(BL6) unknown WT male 00-00-0000
PH02269 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02270 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02271 Munc18-1(BL6) unknown WT male 00-00-0000
PH02272 Munc18-1(BL6) unknown WT male 00-00-0000
PH02274 Munc18-1(BL6) unknown WT male 00-00-0000
PH02275 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02276 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02277 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02305 Munc18-1(BL6) unknown WT male 00-00-0000
PH02306 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02307 Munc18-1(BL6) unknown WT male 00-00-0000
PH02308 Munc18-1(BL6) unknown WT male 00-00-0000
PH02309 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02310 Munc18-1(BL6) unknown WT male 00-00-0000
PH02311 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02312 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02313 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02314 Munc18-1(BL6) unknown WT male 00-00-0000
PH02315 Munc18-1(BL6) unknown WT male 00-00-0000
PH02317 Munc18-1(BL6) unknown WT male 00-00-0000
PH02318 Munc18-1(BL6) unknown WT male 00-00-0000
PH02319 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02320 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02321 Munc18-1(BL6) unknown WT male 00-00-0000
PH02322 Munc18-1(BL6) unknown hetro male 00-00-0000
PH02323 Munc18-1(BL6) unknown WT male 00-00-0000
PH02324 Munc18-1(BL6) unknown WT male 00-00-0000
PH03598 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03599 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03600 Munc18-1(BL6) unknown WT male 00-00-0000
PH03601 Munc18-1(BL6) unknown WT male 00-00-0000
PH03602 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03603 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03604 Munc18-1(BL6) unknown WT male 00-00-0000
PH03605 Munc18-1(BL6) unknown WT male 00-00-0000
PH03606 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03607 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03608 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03609 Munc18-1(BL6) unknown WT male 00-00-0000
PH03610 Munc18-1(BL6) unknown WT male 00-00-0000
PH03611 Munc18-1(BL6) unknown WT male 00-00-0000
PH03612 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03613 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03614 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03615 Munc18-1(BL6) unknown WT male 00-00-0000
PH03616 Munc18-1(BL6) unknown WT male 00-00-0000
PH03617 Munc18-1(BL6) unknown WT male 00-00-0000
PH03618 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03619 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03620 Munc18-1(BL6) unknown hetro male 00-00-0000
PH03621 Munc18-1(BL6) unknown WT male 00-00-0000
PH03622 Munc18-1(BL6) unknown WT male 00-00-0000
PH04314 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04315 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04316 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04317 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04318 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04319 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04320 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04321 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04322 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04323 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04324 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04325 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04326 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04327 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04328 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04329 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04330 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04331 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04332 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04333 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH04334 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04335 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04336 Munc18-1(Floxed) unknown WT male 00-00-0000
PH04337 Munc18-1(Floxed) unknown hetro male 00-00-0000
PH09078 Munc18-1(129Sv) black hetro L 1439 male 09-01-2014
PH09079 Munc18-1(129Sv) black hetro RL 1460 male 09-01-2014
PH09080 Munc18-1(129Sv) black hetro RR 1461 male 09-01-2014
PH09081 Munc18-1(129Sv) black hetro LL 1462 male 09-01-2014
PH09082 Munc18-1(129Sv) black hetro L 1465 male 13-01-2014
PH09083 Munc18-1(129Sv) black hetro RL 1466 male 13-01-2014
PH09084 Munc18-1(129Sv) black WT R 1438 male 09-01-2014
PH09085 Munc18-1(129Sv) black WT RL 1440 male 09-01-2014
PH09086 Munc18-1(129Sv) black WT R 1445 male 09-01-2014
PH09087 Munc18-1(129Sv) black WT L 1446 male 09-01-2014
PH09088 Munc18-1(129Sv) black WT RL 1447 male 09-01-2014
PH09089 Munc18-1(129Sv) black WT - 1457 male 09-01-2014
PH09090 Munc18-1(129Sv) black WT R 1458 male 09-01-2014
PH09091 Munc18-1(129Sv) black WT L 1459 male 09-01-2014
PH09092 Munc18-1(129Sv) black WT R 1464 male 13-01-2014
PH09093 Munc18-1(129Sv) black hetro R 1471 male 15-01-2014
PH09094 Munc18-1(129Sv) black hetro RL 1473 male 15-01-2014
PH09095 Munc18-1(129Sv) black hetro RR 1474 male 15-01-2014
PH09096 Munc18-1(129Sv) black hetro LL 1475 male 15-01-2014
PH09097 Munc18-1(129Sv) black WT R 1479 male 16-01-2014
PH09098 Munc18-1(129Sv) black hetro R 1933 male 27-01-2014
PH09099 Munc18-1(129Sv) black hetro L 1934 male 27-01-2014
PH09100 Munc18-1(129Sv) black WT RL 1935 male 27-01-2014
PH09101 Munc18-1(129Sv) black WT RR 1936 male 27-01-2014
PH13410 Munc18-1(BL6) black hetro 3068 male 05-02-2015 Only LTP in paper
PH13411 Munc18-1(BL6) black WT 22644 male 05-02-2015 Only LTP in paper
PH13412 Munc18-1(BL6) black WT 22645 male 05-02-2015 Only LTP in paper
PH13413 Munc18-1(BL6) black hetro 22084 male 05-02-2015 Only LTP in paper
PH13414 Munc18-1(BL6) black WT 22671 male 05-02-2015 Only LTP in paper
PH13415 Munc18-1(BL6) black WT 22672 male 05-02-2015 Only LTP in paper
PH13416 Munc18-1(BL6) black hetro 23526 male 05-02-2015 Only LTP in paper
PH13417 Munc18-1(BL6) black hetro 23525 male 05-02-2015 Only LTP in paper
PH13418 Munc18-1(BL6) black WT 22623 male 05-02-2015 Only LTP in paper
PH13419 Munc18-1(BL6) black hetro 22629 male 05-02-2015 Only LTP in paper
PH13420 Munc18-1(BL6) black hetro 22630 male 05-02-2015 Only LTP in paper
PH13421 Munc18-1(BL6) black WT 22624 male 05-02-2015 Only LTP in paper
PH13422 Munc18-1(BL6) black hetro 23163 male 05-02-2015 Only LTP in paper
PH13423 Munc18-1(BL6) black WT 22584 male 05-02-2015 Only LTP in paper
PH13424 Munc18-1(BL6) black WT 22585 male 05-02-2015 Only LTP in paper
PH13425 Munc18-1(BL6) black hetro 22598 male 05-02-2015 Only LTP in paper
PH13426 Munc18-1(BL6) black WT 22578 male 05-02-2015 Only LTP in paper
PH13427 Munc18-1(BL6) black WT 22577 male 05-02-2015 Only LTP in paper
PH13428 Munc18-1(BL6) black hetro 22600 male 05-02-2015 Only LTP in paper
PH13429 Munc18-1(BL6) black hetro 22775 male 05-02-2015 Only LTP in paper
PH13430 Munc18-1(BL6) black WT 22782 male 05-02-2015 Only LTP in paper
PH13431 Munc18-1(BL6) black hetro 23162 male 05-02-2015 Only LTP in paper
PH13432 Munc18-1(BL6) black WT 22783 male 05-02-2015 Only LTP in paper
PH13433 Munc18-1(BL6) black hetro 23067 male 05-02-2015 Only LTP in paper
PH13434 Munc18-1(BL6) black hetro 3068 male 05-02-2015
PH13435 Munc18-1(BL6) black WT 22644 male 05-02-2015
PH13436 Munc18-1(BL6) black WT 22645 male 05-02-2015
PH13437 Munc18-1(BL6) black hetro 22084 male 05-02-2015
PH13438 Munc18-1(BL6) black WT 22671 male 05-02-2015
PH13439 Munc18-1(BL6) black WT 22672 male 05-02-2015
PH13440 Munc18-1(BL6) black hetro 23526 male 05-02-2015
PH13441 Munc18-1(BL6) black hetro 23525 male 05-02-2015
PH13442 Munc18-1(BL6) black WT 22623 male 05-02-2015
PH13443 Munc18-1(BL6) black hetro 22629 male 05-02-2015
PH13444 Munc18-1(BL6) black hetro 22630 male 05-02-2015
PH13445 Munc18-1(BL6) black WT 22624 male 05-02-2015
PH13446 Munc18-1(BL6) black hetro 23163 male 05-02-2015
PH13447 Munc18-1(BL6) black WT 22584 male 05-02-2015
PH13448 Munc18-1(BL6) black WT 22585 male 05-02-2015
PH13449 Munc18-1(BL6) black hetro 22598 male 05-02-2015
PH13450 Munc18-1(BL6) black WT 22578 male 05-02-2015
PH13451 Munc18-1(BL6) black WT 22577 male 05-02-2015
PH13452 Munc18-1(BL6) black hetro 22600 male 05-02-2015
PH13453 Munc18-1(BL6) black hetro 22775 male 05-02-2015
PH13454 Munc18-1(BL6) black WT 22782 male 05-02-2015
PH13455 Munc18-1(BL6) black hetro 23162 male 05-02-2015
PH13456 Munc18-1(BL6) black WT 22783 male 05-02-2015
PH13457 Munc18-1(BL6) black hetro 23067 male 05-02-2015
PH13912 Munc18-1(BL6) black hetro 3068 male 05-02-2015 BM reversal
PH13913 Munc18-1(BL6) black WT 22644 male 05-02-2015 BM reversal
PH13914 Munc18-1(BL6) black WT 22645 male 05-02-2015 BM reversal
PH13915 Munc18-1(BL6) black hetro 22084 male 05-02-2015 BM reversal
PH13916 Munc18-1(BL6) black WT 22671 male 05-02-2015 BM reversal
PH13917 Munc18-1(BL6) black WT 22672 male 05-02-2015 BM reversal
PH13918 Munc18-1(BL6) black hetro 23526 male 05-02-2015 BM reversal
PH13919 Munc18-1(BL6) black hetro 23525 male 05-02-2015 BM reversal
PH13920 Munc18-1(BL6) black WT 22623 male 05-02-2015 BM reversal
PH13921 Munc18-1(BL6) black hetro 22629 male 05-02-2015 BM reversal
PH13922 Munc18-1(BL6) black hetro 22630 male 05-02-2015 BM reversal
PH13923 Munc18-1(BL6) black WT 22624 male 05-02-2015 BM reversal
PH13924 Munc18-1(BL6) black hetro 23163 male 05-02-2015 BM reversal
PH13925 Munc18-1(BL6) black WT 22584 male 05-02-2015 BM reversal
PH13926 Munc18-1(BL6) black WT 22585 male 05-02-2015 BM reversal
PH13927 Munc18-1(BL6) black hetro 22598 male 05-02-2015 BM reversal
PH13928 Munc18-1(BL6) black WT 22578 male 05-02-2015 BM reversal
PH13929 Munc18-1(BL6) black WT 22577 male 05-02-2015 BM reversal
PH13930 Munc18-1(BL6) black hetro 22600 male 05-02-2015 BM reversal
PH13931 Munc18-1(BL6) black hetro 22775 male 05-02-2015 BM reversal
PH13932 Munc18-1(BL6) black WT 22782 male 05-02-2015 BM reversal
PH13933 Munc18-1(BL6) black hetro 23162 male 05-02-2015 BM reversal
PH13934 Munc18-1(BL6) black WT 22783 male 05-02-2015 BM reversal
PH13935 Munc18-1(BL6) black hetro 23067 male 05-02-2015 BM reversal
PH15280 Munc18-1(Floxed) black WT 35980 male 25-07-2015
PH15281 Munc18-1(Floxed) black WT 36007 male 21-07-2015
PH15282 Munc18-1(Floxed) black WT 36022 male 25-07-2015
PH15283 Munc18-1(Floxed) black WT 36024 male 25-07-2015
PH15284 Munc18-1(Floxed) black WT 36025 male 25-07-2015
PH15285 Munc18-1(Floxed) black WT 36032 male 21-07-2015
PH15286 Munc18-1(Floxed) black WT 36033 male 21-07-2015
PH15287 Munc18-1(Floxed) black WT 36035 male 21-07-2015
PH15288 Munc18-1(Floxed) black WT 36048 male 22-07-2015
PH15289 Munc18-1(Floxed) black WT 36051 male 22-07-2015
PH15290 Munc18-1(Floxed) black WT 36053 male 22-07-2015
PH15291 Munc18-1(Floxed) black WT 36056 male 21-07-2015
PH15292 Munc18-1(Floxed) black hetro 35979 male 25-07-2015
PH15293 Munc18-1(Floxed) black hetro 36008 male 21-07-2015
PH15294 Munc18-1(Floxed) black hetro 36018 male 22-07-2015
PH15295 Munc18-1(Floxed) black hetro 36023 male 25-07-2015
PH15296 Munc18-1(Floxed) black hetro 36026 male 25-07-2015
PH15297 Munc18-1(Floxed) black hetro 36031 male 21-07-2015
PH15298 Munc18-1(Floxed) black hetro 36034 male 21-07-2015
PH15299 Munc18-1(Floxed) black hetro 36036 male 21-07-2015
PH15300 Munc18-1(Floxed) black hetro 36049 male 22-07-2015
PH15301 Munc18-1(Floxed) black hetro 36050 male 22-07-2015
PH15302 Munc18-1(Floxed) black hetro 36052 male 22-07-2015
PH15303 Munc18-1(Floxed) black hetro 36057 male 21-07-2015
PH15304 Munc18-1(Floxed) black WT 35980 male 25-07-2015
PH15305 Munc18-1(Floxed) black WT 36007 male 21-07-2015
PH15306 Munc18-1(Floxed) black WT 36022 male 25-07-2015
PH15307 Munc18-1(Floxed) black WT 36024 male 25-07-2015
PH15308 Munc18-1(Floxed) black WT 36025 male 25-07-2015
PH15309 Munc18-1(Floxed) black WT 36032 male 21-07-2015
PH15310 Munc18-1(Floxed) black WT 36033 male 21-07-2015
PH15311 Munc18-1(Floxed) black WT 36035 male 21-07-2015
PH15312 Munc18-1(Floxed) black WT 36048 male 22-07-2015
PH15313 Munc18-1(Floxed) black WT 36051 male 22-07-2015
PH15314 Munc18-1(Floxed) black WT 36053 male 22-07-2015
PH15315 Munc18-1(Floxed) black WT 36056 male 21-07-2015
PH15316 Munc18-1(Floxed) black hetro 35979 male 25-07-2015
PH15317 Munc18-1(Floxed) black hetro 36008 male 21-07-2015
PH15318 Munc18-1(Floxed) black hetro 36018 male 22-07-2015
PH15319 Munc18-1(Floxed) black hetro 36023 male 25-07-2015
PH15320 Munc18-1(Floxed) black hetro 36026 male 25-07-2015
PH15321 Munc18-1(Floxed) black hetro 36031 male 21-07-2015
PH15322 Munc18-1(Floxed) black hetro 36034 male 21-07-2015
PH15323 Munc18-1(Floxed) black hetro 36036 male 21-07-2015
PH15324 Munc18-1(Floxed) black hetro 36049 male 22-07-2015
PH15325 Munc18-1(Floxed) black hetro 36050 male 22-07-2015
PH15326 Munc18-1(Floxed) black hetro 36052 male 22-07-2015
PH15327 Munc18-1(Floxed) black hetro 36057 male 21-07-2015